Regulatory and Enterotoxin Gene Expression and Enterotoxins Production in Staphylococcus aureus FRI913 Cultures Exposed to a Rotating Magnetic Field and trans-Anethole
Kwiatkowski Paweł, Aleksandra Tabiś, Karol Fijałkowski, Helena Masiuk, Łukasz Łopusiewicz, Agata Pruss, Monika Sienkiewicz, Marcin Wardach, Mateusz Kurzawski, Sebastian Guenther, Jacek Bania, Barbara Dołęgowska, Iwona Wojciechowska-Koszko
International Journal of Molecular Sciences
Ministerial score = 140.0
Journal Impact Factor (2021) = 6.208 (Q1)
The study aimed to examine the influence of a rotating magnetic field (RMF) of two different frequencies (5 and 50 Hz) on the expression of regulatory (agrA, hld, rot) and staphylococcal enterotoxin (SE—sea, sec, sel) genes as well as the production of SEs (SEA, SEC, SEL) by the Staphylococcus aureus FRI913 strain cultured on a medium supplemented with a subinhibitory concentration of trans-anethole (TA). Furthermore, a theoretical model of interactions between the bacterial medium and bacterial cells exposed to RMF was proposed. Gene expression and SEs production were measured using quantitative real-time PCR and ELISA techniques, respectively. Based on the obtained results, it was found that there were no significant differences in the expression of regulatory and SE genes in bacteria simultaneously cultured on a medium supplemented with TA and exposed to RMF at the same time in comparison to the control (unexposed to TA and RMF). In contrast, when the bacteria were cultured on a medium supplemented with TA but were not exposed to RMF or when they were exposed to RMF of 50 Hz (but not to TA), a significant increase in agrA and sea transcripts as compared to the unexposed control was found. Moreover, the decreased level of sec transcripts in bacteria cultured without TA but exposed to RMF of 50 Hz was also revealed. In turn, a significant increase in SEA and decrease in SEC and SEL production was observed in bacteria cultured on a medium supplemented with TA and simultaneously exposed to RMFs. It can be concluded, that depending on SE and regulatory genes expression as well as production of SEs, the effect exerted by the RMF and TA may be positive (i.e., manifests as the increase in SEs and/or regulatory gene expression of SEs production) or negative (i.e., manifests as the reduction in both aforementioned features) or none.
DOI:10.3390/ijms23116327
- News
- University
- About university
- Authorities
- Structure
- Campus map
- 70 years of UPWr
- Employee Code of Ethics
- International Relations Office
- Library
- 2024 election
- University events
- Students
- Academic calendar for students
- The study regulations
- Accommodation
- Accident Insurance
- Student guide
- Erasmus+
- Student activities
- Young Minds Project
- Study Abroad
- Centre for Foreign Languages, Humanities and Social Sciences
- CEEPUS
- Library
- UPWr Summer School
- Support for Ukrainian and Belarusian students of UPWr
- Scholarships
- Research
- Leading scientific disciplines
- Leading Research Groups
- AgrEn – Agriculture-Environment-Natural Resources
- ASc4Future – Animal Science for Future
- BioActiv – Biocatalysis and biological activity
- BioDivRO – Biodiversity and sustainable development in Agriculture and Horticulture
- BioTech@Life – Biotechnology for life and industry
- DroPOWER – Poultry – from stable to table
- Food&Health – Food and Health
- Inno-WET – Innovative diagnostics and veterinary therapy
- Inoter – Smart and modern technologies in agriculture
- LandArch – Landscape architecture
- OncoVet - Veterinary Oncology
- Plants4FOOD – Plants Processing & Technology for Bioactive Food
- SCR – Sustainable Cities and Regions
- SPACEOS– Space And Close Earth Observation Sciences
- THETA – The Biostatistic Group
- WBVG – Waste and Biomass Valorization Group
- WCE – Water-Climate-Environment
- Research projects
- UPWr Base of Knowledge
- Research papers of the week
- Research papers of the week – September 30, 2024
- Research papers of the week – September 23, 2024
- Research papers of the week – September 16, 2024
- Research papers of the week – September 9, 2024
- Research papers of the week – September 2, 2024
- Research papers of the week – August 26, 2024
- Research papers of the week – August 19, 2024
- Research papers of the week – August 12, 2024
- Research papers of the week – August 5, 2024
- Research papers of the week – July 29, 2024
- Research papers of the week – July 22, 2024
- Research papers of the week – July 15, 2024
- Research papers of the week – July 8, 2024
- Research papers of the week – July 1, 2024
- Research papers of the week – June 24, 2024
- Research papers of the week – June 17, 2024
- Research papers of the week – June 10, 2024
- Research papers of the week – June 3, 2024
- Research papers of the week – May 27, 2024
- Research papers of the week – May 20, 2024
- Research papers of the week – May 13, 2024
- Research papers of the week – May 6, 2024
- Research papers of the week – April 29, 2024
- Research papers of the week – April 22, 2024
- Research papers of the week – April 15, 2024
- Research papers of the week – April 8, 2024
- Research papers of the week – April 1, 2024
- Research papers of the week – March 25, 2024
- Research papers of the week – March 18, 2024
- Research papers of the week – March 11, 2024
- Research papers of the week – March 4, 2024
- Research papers of the week – February 26, 2024
- Research papers of the week – February 19, 2024
- Research papers of the week – February 12, 2024
- Research papers of the week – February 5, 2024
- Research papers of the week – January 29, 2024
- Research papers of the week – January 22, 2024
- Research papers of the week – January 15, 2024
- Research papers of the week – January 8, 2024
- Research papers of the week – January 1, 2024
- Research papers of the week – December 25, 2023
- Research papers of the week – December 18, 2023
- Research papers of the week – December 11, 2023
- Research papers of the week – December 4, 2023
- Research papers of the week – November 27, 2023
- Research papers of the week – November 20, 2023
- Research papers of the week – November 13, 2023
- Research papers of the week – November 6, 2023
- Research papers of the week – October 30, 2023
- Research papers of the week – October 23, 2023
- Research papers of the week – October 16, 2023
- Research papers of the week – October 9, 2023
- Research papers of the week – October 2, 2023
- Research papers of the week – September 25, 2023
- Research papers of the week – September 18, 2023
- Research papers of the week – September 11, 2023
- Research papers of the week – September 4, 2023
- Research papers of the week – August 28, 2023
- Research papers of the week – August 21, 2023
- Research papers of the week – August 14, 2023
- Research papers of the week – August 7, 2023
- Research papers of the week – July 31, 2023
- Research papers of the week – July 24, 2023
- Research papers of the week – July 17, 2023
- Research papers of the week – July 10, 2023
- Research papers of the week – July 3, 2023
- Research papers of the week – June 26, 2023
- Research papers of the week – June 19, 2023
- Research papers of the week – June 12, 2023
- Research papers of the week – June 5, 2023
- Research papers of the week – May 29, 2023
- Research papers of the week – May 22, 2023
- Research papers of the week – May 15, 2023
- Research papers of the week – May 8, 2023
- Research papers of the week – May 4, 2023
- Research papers of the week – April 24, 2023
- Research papers of the week – April 17, 2023
- Research papers of the week – April 11, 2023
- Research papers of the week – April 3, 2023
- Research papers of the week – March 27, 2023
- Research papers of the week – March 20, 2023
- Research papers of the week – March 13, 2023
- Research papers of the week – March 6, 2023
- Research papers of the week – February 27, 2023
- Research papers of the week – February 20, 2023
- Research papers of the week – February 13, 2023
- Research papers of the week – February 6, 2023
- Research papers of the week – January 30, 2023
- Research papers of the week – January 23, 2023
- Research papers of the week – January 16, 2023
- Research papers of the week – January 9, 2023
- Research papers of the week – January 2, 2023
- Research papers of the week – December 26, 2022
- Research papers of the week – December 19, 2022
- Research papers of the week – December 12, 2022
- Research papers of the week – December 5, 2022
- Research papers of the week – November 28, 2022
- Research papers of the week – November 21, 2022
- Research papers of the week – November 14, 2022
- Research papers of the week – November 7, 2022
- Research papers of the week – October 31, 2022
- Research papers of the week – October 24, 2022
- Research papers of the week – October 17, 2022
- Research papers of the week – October 3, 2022
- Research papers of the week – September 26, 2022
- Research papers of the week – September 20, 2022
- Research papers of the week – September 12, 2022
- Research papers of the week – September 5, 2022
- Research papers of the week – August 29, 2022
- Research papers of the week – August 22, 2022
- Research papers of the week – August 15, 2022
- Research papers of the week – August 8, 2022
- Research papers of the week – August 1, 2022
- Research papers of the week - July 25, 2022
- Research papers of the week - July 18, 2022
- Research papers of the week - July 11, 2022
- Research papers of the week - July 4, 2022
- Research papers of the week - June 27, 2022
- Research papers of the week - June 20, 2022
- Research papers of the week - June 13, 2022
- Research papers of the week - June 6, 2022
- Research papers of the week - May 30, 2022
- Research papers of the week – May 22, 2022
- Research papers of the week - May 16, 2022
- Research papers of the week - May 9, 2022
- Research papers of the week - May, 4 2022
- Research papers of the week – April, 25 2022
- Research papers of the week – April 18, 2022
- Research papers of the week – April 11, 2022
- Research papers of the week – April 4, 2022
- Research papers of the week - March 28, 2022
- Research papers of the week - March 21 , 2022
- Research papers of the week - March 14, 2021
- Research papers of the week - March 7th, 2022
- Research papers of the week - Feburary 28, 2022
- Research papers of the week - Feburary 21, 2022
- Research papers of the week - Feburary 14, 2022
- Research papers of the week - Feburary 6, 2022
- Research papers of the week - January 31, 2022
- Research papers of the week – January 24, 2022
- Research papers of the week – January 17, 2022
- Research papers of the week – January 10, 2022
- Research papers of the week – January 03, 2022
- Research papers of the week – December 27, 2021
- Research papers of the week – December 20, 2021
- Research papers of the week – December 13, 2021
- Research papers of the week – December 6, 2021
- Research papers of the week – November 29, 2021
- Research papers of the week – November 22, 2021
- Research papers of the week – November 16, 2021
- Research papers of the week – November 9, 2021
- Research papers of the week – November 2, 2021
- Research papers of the week – October 25, 2021
- Research papers of the week – October 18, 2021
- Research papers of the week – October 11, 2021
- Research papers of the week – October 4, 2021
- Research papers of the week – September 27, 2021
- Research papers of the week – September 20, 2021
- Research papers of the week – September 13, 2021
- Research papers of the week – September 6, 2021
- Research papers of the week – August 30, 2021
- Research papers of the week – August 23, 2021
- Research papers of the week – August 16, 2021
- Research papers of the week – August 9, 2021
- Research papers of the week – August 2, 2021
- Research papers of the week – July 26, 2021
- Research papers of the week – July 19, 2021
- Research papers of the week – July 12, 2021
- Research papers of the week – July 5, 2021
- Research papers of the week – June 28, 2021
- Research papers of the week – June 14, 2021
- Research papers of the week – June 7, 2021
- Research papers of the week – May 31, 2021
- Research papers of the week – May 24, 2021
- Research papers of the week – May 17, 2021
- Research papers of the week – May 10, 2021
- Research papers of the week – May 3, 2021
- Research papers of the week – April 26, 2021
- Research papers of the week – April 19, 2021
- Research papers of the week – April 12, 2021
- Research papers of the week – April 5, 2021
- Research papers of the week – March 29, 2021
- Research papers of the week – March 22, 2021
- Research papers of the week – March 15, 2021
- Research papers of the week – March 8, 2021
- Research papers of the week – March 1, 2021
- Research papers of the week – February 22, 2021
- Research papers of the week – February 15, 2021
- Research papers of the week – February 8, 2021
- Research papers of the week – February 1, 2021
- Research papers of the week – January 25, 2021
- Research papers of the week – January 18, 2021
- Research papers of the week – January 11, 2021
- Research papers of the week – January 4, 2021
- Research papers of the week – December 21, 2020
- Research papers of the week – December 14, 2020
- Research papers of the week – December 7, 2020
- Research papers of the week – November 30, 2020
- Research papers of the week – November 23, 2020
- Research papers of the week – November 16, 2020
- Research papers of the week – November 9, 2020
- Research papers of the week – November 2, 2020
- Research papers of the week – October 26, 2020
- Research papers of the week – October 19, 2020
- Research papers of the week – October 12, 2020
- Research papers of the week – October 5, 2020
- Research papers of the week – September 28, 2020
- UPWr Doctoral School
- Research collaboration offers
- Post-doc offer
- Offers for UA reseachers
- Offers for UA reseachers – Leading Research Groups (UPWr)
- Innovative diagnostics and veterinary therapy – Inno-WET
- Agriculture-Environment-Natural Resources – AgrEn
- Biotechnology for life and industry – BioTech@Life
- Biocatalysis and biological activity – BioActiv
- Reg-Med-Lab – Marycz Lab
- Sustainable Cities and Regions – SCR
- Space And Close Earth Observation Sciences – SpaceOS
- General veterinary, preclinical and hygienic science – WET-PPH
- Waste and Biomass Valorization Group – WBVG
- Offers for UA researchers outside of UPWr
- Programmes and Grants
- Offers for UA reseachers – Leading Research Groups (UPWr)
- The Excellence Initiative – Research University
- European Charter for Researchers
- Collaboration
- Admission
- For employees